کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2815906 | 1159900 | 2015 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Isoform-specific imprinting of the MEST gene in porcine parthenogenetic fetuses
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کلمات کلیدی
GAPDHCOBRADMRBSPBisulfite sequencing PCR - PCR توالی بیسولفیتq-PCR - Q-PCRIsoform - ایسوفورcon - باcombined bisulfite restriction analysis - تجزیه و تحلیل محدود بیسولفیتMEST - ترینDifferentially methylated region - منطقه متخلخل متفاوتی داردimprinting - نقش پذیری quantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیparthenogenetic - پارتن ژنتیکParthenogenesis - پارتیونوژنزControl - کنترلglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژنازPorcine - گوشت خوک
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Aberrant expression of imprinted genes is the main reason for developmental retardation in mammalian parthenogenetic fetuses. Mesoderm specific transcript (MEST) is a maternally imprinted gene that is linked to cancer and is necessary for normal early embryonic development. Tissue and isoform-specific imprinting of MEST have been identified in humans and mice, but have not yet been identified in pigs. In this study, the three isoforms of porcine MEST were identified using the GenBank and Ensembl sequence databases. Then, we determined MEST isoform-specific mRNA expression and methylation levels by quantitative real-time PCR (qRT-PCR) and bisulfite sequencing PCR analysis (BSP) between porcine parthenogenetic (PA) and control (Con) fetuses, respectively. Altogether, our results demonstrated that the expression of MEST-1A and MEST-1B has no evident differences between PA and Con groups; however, there is no expression of MEST-1C in PA fetuses. In addition, the results of BSP showed that the hypermethylation of exon 1c of MEST-1C was observed in PA samples. Thus, we suggested that MEST-1C was isoform-specific imprinting, which may be contributed to differential methylation status of exon 1c in porcine fetuses.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 558, Issue 2, 10 March 2015, Pages 287-290
Journal: Gene - Volume 558, Issue 2, 10 March 2015, Pages 287-290
نویسندگان
Xiaolan Li, Nan Song, Dongxu Wang, Xiaolei Han, Qingyan Lv, Hongsheng Ouyang, Zhanjun Li,