کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2840604 1165340 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
trehalase-2 protein contributes to trehalase activity enhanced by diapause hormone in developing ovaries of the silkworm, Bombyx mori
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش حشره شناسی
پیش نمایش صفحه اول مقاله
trehalase-2 protein contributes to trehalase activity enhanced by diapause hormone in developing ovaries of the silkworm, Bombyx mori
چکیده انگلیسی

Diapause hormone (DH) targets developing ovaries in female pupae to induce embryonic diapause immediately after completion of mesoderm segregation of the silkworm, Bombyx mori. At the same time, DH enhances trehalase activity on the oolemma, which leads to higher concentrations of glycogen in oocytes through the stimulated incorporation of hemolymph trehalose. In B. mori, the treh-1 and -2 genes encoding soluble trehalase (68 kDa) and integral-membrane trehalase (74 kDa) have been isolated. DH stimulates mRNA expression of both of these genes. In this study, we aimed to clarify whether ovarian trehalase originates from Treh-1 or Treh-2. Western blotting of the developing ovaries showed positive bands in the membrane-bound fraction, containing trehalase activity, only with antibodies against Treh-1&2 and Treh-2, but not Treh-1, irrespective of nondiapause or diapause egg-producers. The intensities of the positively stained 74 kDa bands were increased approximately 4-fold in ovaries from pupae with intact subesophageal ganglion (SG, a unique DH-biosynthesizing organ), and from pupae that were injected with DH at the middle pupal stage after their SGs were removed on the day of pupation. Furthermore, quantitative real-time PCR data showed that in developing ovaries, copy number of treh-2 mRNA per one copy of rp49 mRNA was approximately 1000-fold higher than that of treh-1 mRNA. These results demonstrate that trehalase activities enhanced by DH originate mainly from treh-2 protein regulated at the transcriptional level.

Figure optionsDownload as PowerPoint slideResearch highlights▶ Diapause hormone (DH) enhances trehalase activity in ovaries of Bombyx mori female pupae. ▶ Western blotting analysis was carried out, using antibodies to soluble trehalase (68 kDa, Treh-1) and integral-membrane trehalase (74 kDa, Treh-2). ▶ In ovaries treated with DH, the intensities of 74 kDa bands were increased, and amount of treh-2 mRNA was about 1000-fold higher than treh-1 mRNA. ▶ Trehalase activity enhanced by DH was shown to originate mainly from treh-2 protein.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Insect Physiology - Volume 57, Issue 5, May 2011, Pages 608–613
نویسندگان
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