کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2843650 | 1571107 | 2007 | 7 صفحه PDF | دانلود رایگان |
Organisms react to cellular stress by inducing the synthesis of heat-shock proteins (Hsp). One such protein is the mitochondrial 70-kDa Hsp (mHsp70). The expression of mHsp70 in organisms that undergo stress in their natural habitat is unknown. We used a biochemical approach enabling to identify an mHsp70 from the sea anemone Anemonia viridis, which is abundant in highly fluctuating marine habitats. Antibodies raised specifically against yeast mHsp70 recognized a 70-kDa protein from A. viridis. We found that A. viridis mHsp70 is constitutively expressed at 22–23 °C, but over-expressed upon exposure to heat shock (31 °C) or to temperature fluctuations, suggesting that mHsp70 plays a significant role in adaptation of sessile marine invertebrates to highly fluctuating environmental conditions. Using an affinity column we were able to obtain a partially purified fraction of this protein. Partial amino acid sequences proved that the purified Hsp70 functions in the mitochondria.
Journal: Journal of Thermal Biology - Volume 32, Issues 7–8, October–December 2007, Pages 367–373