کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3051964 1579901 2016 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Anti-epileptic drugs and bone loss: Phenytoin reduces pro-collagen I and alters the electrophoretic mobility of osteonectin in cultured bone cells
ترجمه فارسی عنوان
داروهای ضدصرع و از دست دادن استخوان: فنیتوین باعث کاهش پرولا کلاژن I و تحرک الکتروفورتیک استونکتین در سلول های استخوانی کشت شده
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی عصب شناسی
چکیده انگلیسی


• Phenytoin, like valproate, reduces collagen I protein production by osteoblast-like cells.
• Unlike valproate, phenytoin does not reduce osteonectin protein production but instead, alters the electrophoretic mobility of osteonectin.
• Perturbation of these important bone proteins is a possible mechanism to explain bone loss following long-term treatment with phenytoin.

Phenytoin is an antiepileptic drug used in the management of partial and tonic-clonic seizures. In previous studies we have shown that valproate, another antiepileptic drug, reduced the amount of two key bone proteins, pro-collagen I and osteonectin (SPARC, BM-40), in both skin fibroblasts and cultured osteoblast-like cells. Here we show that phenytoin also reduces pro-collagen I production in osteoblast-like cells, but does not appear to cause a decrease in osteonectin message or protein production. Instead, a 24 h exposure to a clinically relevant concentration of phenytoin resulted in a dose-dependent change in electrophoretic mobility of osteonectin, which was suggestive of a change in post-translational modification status. The perturbation of these important bone proteins could be one of the mechanisms to explain the bone loss that has been reported following long-term treatment with phenytoin.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Epilepsy Research - Volume 122, May 2016, Pages 97–101
نویسندگان
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