Investigation on Endogenous Metabolites in Pancreas of Diabetic Rats after Treatment by Genipin through 1H-NMR-based Metabolomic Profiles

ObjectiveTo investigate the change rules of endogenous metabolites in pancreas of the diabetic rats, and to explore the mechanism of genipin treatment for diabetes rats.MethodsMetabolomic method based on 1H-NMR was applied, the diabetic rat model was prepared by ip injecting alloxan, and the high-, mid-, and low-dose genipin or metformin hydrochloride was ig injected as well as the rats in control and model groups were given the same volume of normal saline for 2 weeks. The pancreases of rats were collected and 1H-NMR test was conducted, the metabolomic technology was adopted to analyze the endogenous metabolite changes in pancreas.ResultsThe high-dose genipin possessed a better hypoglycemic effect, which could increase the contents of isoleucine, phosphatidylcholine, and phosphatidylethanolamine, and significantly reduce the contents of lactic acid, alanine, glutamine, aspartic acid, and creatine in pancreas of diabetic rats.ConclusionThis study provided a theoretical basis for further exploration on the pathogenesis of diabetes and the mechanism of genipin for treatment of diabetes.