Effect on vascular permeability of a self-etching adhesive system containing an antimicrobial quaternary ammonium polymer QAMP into subcutaneous tissue of rats

• Data suggest that self-etching adhesive systems containing QAMP are safe for clinical use.
• QAMP provide lower effect on the exudative phase of the inflammatory process.
• Laser-Doppler flowmetric results were consistent with those observed for the Evans blue method.
• Investigations must be carried out to assess the effects on inflammatory cell for longer periods.

ObjectiveThis study investigated the effect of a self-etching adhesive system containing an antimicrobial quaternary ammonium methacrylate polymer (QAMP) on the vascular permeability using Evans blue and laser-Doppler flowmetric methods.DesignForty rats were anesthetized and divided into groups: saline solution; Clearfil™ Protect Bond; Clearfil™ SE Bond; Clearfil™ SE Bond containing QAMP. Injections of Evans blue were administrated intravenously and the substances were injected intradermally. Immediately before injection and after 3 and 6 h, was evaluated the blood flow. The animals were killed 3 and 6 h after injection. The dorsal skin was dissected and experimental sites were obtained. The vascular permeability was evaluated by dye extravasation area, the dye was extracted and absorbance measured.ResultsConcerning the Evans blue method, Clearfil™ SE Bond containing QAMP showed an extravasation area statistically similar to Clearfil™ SE Bond and lower than Clearfil™ Protect Bond. No statistical difference was verified among experimental groups for the amount of dye extracted. Clearfil™ SE Bond containing QAMP provided better laser-Doppler flowmetric parameters than Clearfil™ Protect Bond immediately and 3 h after injection.ConclusionThe QAMP had lower effect on the exudative phase of the inflammatory process.