کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3335900 | 1213549 | 2010 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
CFSE flow cytometric quantification of lymphocytic proliferation in extracorporeal photopheresis: Use for quality control
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کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
هماتولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Quality control is essential to validate extracorporeal photopheresis (ECP) processes. There is just one protocol based on PHA-induced proliferation. Since it involves the use of radioactive thymidine, we developed another technique using CFSE labeling. We compared the two tests in a paired series including 18 procedures. The thymidine test was valid. Once proliferation was obtained (10 patients out of 13), the CFSE test was in close agreement with it. In particular, two cases of residual proliferation after ECP were simultaneously detected by both techniques. Only the CFSE test allows targeted analysis of lymphocytes, thus identifying a surviving lymphocytic sub-population.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Transfusion and Apheresis Science - Volume 42, Issue 1, February 2010, Pages 11–19
Journal: Transfusion and Apheresis Science - Volume 42, Issue 1, February 2010, Pages 11–19
نویسندگان
Bertrand Evrard, Annie Dosgilbert, Nathalie Jacquemot, François Demeocq, Thibault Gilles, Jacques Chassagne, Marc Berger, Arlette Tridon,