کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3349558 | 1216357 | 2016 | 4 صفحه PDF | دانلود رایگان |
Little information has been reported regarding the regulatory region of the HLA-E gene. Only a proximal segment (300 bp immediately before exon 1) has been described at IMGT/HLA database. We aimed to evaluate the genetic diversity of the promoter region by PCR amplification and DNA sequencing. We observed a pattern of sequencing interruption in the same position in all samples, suggesting the presence of a secondary structure hampering the DNA polymerase sliding during the sequencing process, which was confirmed after bioinformatics analysis. Considering the promoter region evaluated (nucleotide −440 to −1), only three variation sites were found, including one new variation site at position −104, and the concomitant deletions already described at positions −187 and −186. We concluded that the promoter region of the HLA-E gene presents few and rare variable sites in this population sample; however, the double-stranded branch formation hampered the evaluation of the distant promoter by conventional sequencing.
Journal: Human Immunology - Volume 77, Issue 2, February 2016, Pages 172–175