کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3349943 1216368 2015 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Higher TNFα responses in young males compared to females are associated with attenuation of monocyte adenylyl cyclase expression
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Higher TNFα responses in young males compared to females are associated with attenuation of monocyte adenylyl cyclase expression
چکیده انگلیسی

Tumor necrosis factor α (TNFα) expression is strongly attenuated by the intracellular signaling mediator cyclic adenosine monophosphate (cAMP), which is synthesized by adenylyl cyclase (AC) enzymes. We have compared AC regulation and TNFα production in male and female monocytes, and characterized the role of monocyte AC isoforms in TNFα regulation.Males and females, age groups 20–30 years and 50–70 years donated blood for this study. In lipopolysaccharide-stimulated blood from young male donors, we observed significantly higher TNFα responses (6 h, p = 0.03) compared to females of the same age, a difference not observed in the older donors. Rapid down-regulation of the monocyte AC isoforms AC4, AC7 and AC9 were observed in young males. AC-directed siRNA experiments in the human monocyte cell line THP-1 demonstrated that AC7 and AC9 knock-down significantly induced TNFα release (p = 0.01 for both isoforms). These data indicate that the stronger TNFα-responses in young males may be partly associated with male-specific down-regulation of adenylyl cyclases.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Human Immunology - Volume 76, Issue 6, June 2015, Pages 427–430
نویسندگان
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