کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3426105 | 1227315 | 2007 | 11 صفحه PDF | دانلود رایگان |
We studied the interaction of RV with human peripheral blood mononuclear cells (PBMC) from adult volunteers. After exposure of PBMC to rhesus RV (RRV), T and B lymphocytes, NK cells, monocytes, and myeloid and plasmacytoid dendritic cells expressed RV non-structural proteins, at variable levels. Expression of these RV proteins was abolished if infection was done in the presence of anti-VP7 neutralizing antibodies or 10% autologous serum. Supernatants of RRV exposed PBMC contained TNF-α, IL-6, IFN-α, IFN-γ, IL-2 and IL-10. Plasmacytoid DC were found to be the main source of IFN-α production, and in their absence the production of IFN-γ and the frequency of RV specific T cells that secrete IFN-γ diminished. Finally, we could not detect RV-antigen associated with the PBMC or expression of RV non-structural proteins in PBMC of acutely RV-infected children. Thus, although PBMC are susceptible to the initial steps of RV infection, most PBMC of children with RV-gastroenteritis are not infected.
Journal: Virology - Volume 366, Issue 1, 15 September 2007, Pages 174–184