کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4011096 1602574 2016 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Murine fundus fluorescein angiography: An alternative approach using a handheld camera
ترجمه فارسی عنوان
آنژیوگرافی فلورسئین فوندوس موش: با استفاده از یک روش جایگزین یک دوربین دستی
کلمات کلیدی
تصویربرداری؛ ماکولا؛ شبکیه چشم؛ آنژیوگرافی؛ مدل حیوانی تجربی
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی و میکروب شناسی (عمومی)
چکیده انگلیسی


• Adaptation for efficient, cost-effective rodent fundus fluorescein angiography.
• Handheld, lightweight unit: allows flexibility of position for the unpredictable sedated mouse, and ease of transport to facility locations.
• Securely attached Indirect Lens Holder for 90 diopter lens: facilitates stability of focus for capturing the delicate central murine fundus.
• A seamless recording device: permits exceptional mouse retinal vascular imagery.

In today’s modern pharmacologic approach to treating sight-threatening retinal vascular disorders, there is an increasing demand for a compact, mobile, lightweight and cost-effective fluorescein fundus camera to document the effects of antiangiogenic drugs on laser-induced choroidal neovascularization (CNV) in mice and other experimental animals. We have adapted the use of the Kowa Genesis Df Camera to perform Fundus Fluorescein Angiography (FFA) in mice. The 1 kg, 28 cm high camera has built-in barrier and exciter filters to allow digital FFA recording to a Compact Flash memory card. Furthermore, this handheld unit has a steady Indirect Lens Holder that firmly attaches to the main unit, that securely holds a 90 diopter lens in position, in order to facilitate appropriate focus and stability, for photographing the delicate central murine fundus. This easily portable fundus fluorescein camera can effectively record exceptional central retinal vascular detail in murine laser-induced CNV, while readily allowing the investigator to adjust the camera’s position according to the variable head and eye movements that can randomly occur while the mouse is optimally anesthetized. This movable image recording device, with efficiencies of space, time, cost, energy and personnel, has enabled us to accurately document the alterations in the central choroidal and retinal vasculature following induction of CNV, implemented by argon-green laser photocoagulation and disruption of Bruch’s Membrane, in the experimental murine model of exudative macular degeneration.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Eye Research - Volume 148, July 2016, Pages 74–78
نویسندگان
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