کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4012755 | 1261207 | 2006 | 4 صفحه PDF | دانلود رایگان |
Leukocyte accumulation in the retina is thought to play a key role in the early stage of diabetic retinopathy. Leukocyte-endothelial adhesion is important for leukostasis, and high glucose levels may influence it. However, few studies have demonstrated leukocyte-endothelial adhesion using cultured human retinal endothelial cells (HRECs) after high glucose exposure. We report herein the results of neutrophil-endothelial adhesion assay after high glucose exposure. A high concentration of glucose (46.1 mM for 48 h) significantly increased neutrophil adhesion to HRECs as compared to controls. This phenomenon was blocked by PKC inhibitor and anti-intercellular adhesion molecule-1 antibody. Hyperosmolarity did not affect neutrophil-endothelial adhesion. These results suggested that glucose enhances neutrophil adhesion to HRECs through surface expression of ICAM-1 via activation of the PKC pathway.
Journal: Experimental Eye Research - Volume 82, Issue 1, January 2006, Pages 179–182