کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
4311988 1289895 2017 5 صفحه PDF سفارش دهید دانلود کنید
عنوان انگلیسی مقاله
Acute alcohol exposure increases tyrosine hydroxylase protein expression and dopamine synthesis in zebrafish
ترجمه فارسی عنوان
مواجهه شدید الکل، بیان پروتئین هیدروکسیلاز تیروزین و سنتز دوپامین در گورخرماهی را افزایش می دهد
کلمات کلیدی
هیدروکسیلاز تیروزین؛ دوپامین؛ الکل؛ فعالیت حرکتی؛ گورخرماهی
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب رفتاری
چکیده انگلیسی


• Alcohol exposure increases dopamine and DOPAC.
• Alcohol exposure increases tyrosine hydroxylase expression.
• Alcohol exposure increases tyrosine hydroxlase activity.

Zebrafish have become a popular animal model for investigating the effects of alcohol on the brain and behaviour. Acute exposure to alcohol has been shown to alter dopaminergic signalling in zebrafish, but the underlying mechanisms have not been well defined. In the current study, we characterize the effects of alcohol on the zebrafish dopaminergic system by focusing on tyrosine hydroxylase, the rate-limiting enzyme in dopamine synthesis. Using western blot analysis, we demonstrate that a 60 min exposure to 1% alcohol increases tyrosine hydroxylase protein expression in the zebrafish brain. Enzymatic activity assays confirmed that alcohol also increases tyrosine hydroxylase enzymatic activity, whereas HPLC analysis demonstrated increased levels of whole-brain dopamine and its metabolite DOPAC. In addition to activation of the dopaminergic system, behavioural analysis revealed accompanying increase of distance traveled following 1% alcohol exposure. These findings suggest that acute alcohol exposure elevates dopamine synthesis via increased tyrosine hydroxylase protein expression. Our results support the hypothesis that alcohol alters dopaminergic signalling in the zebrafish brain in a similar manner as compared to mammals.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Behavioural Brain Research SreeTestContent1 - Volume 317, 15 January 2017, Pages 237–241
نویسندگان
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