کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4332958 | 1292916 | 2006 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Relevance of astrocytic activation to reductions of astrocytic GABAA receptors
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
MCA[Cl−]iS100BFCSECLEGABATMCAONOSRT-PCRDMEMTPBSGFAPPBSFITCIgGDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده Dulbeccoγ-aminobutyric acid - اسید γ-آمینوبوتیریکtransient middle cerebral artery occlusion - انسداد شریان مغزی میانی متناوبIschemia - ایسکمیimmunoglobulin G - ایمونوگلوبولین GCNS - دستگاه عصبی مرکزیDIV - دیوdays in vitro - روز in vitrofetal calf serum - سرم گوساله جنینcentral nervous system - سیستم عصبی مرکزیmiddle cerebral artery - شریان مغزی میانیintracellular Cl− concentration - غلظت کلسیم داخل سلولیfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریnitric oxide synthase - نیتریک اکسید سنتازreverse-transcription polymerase chain reaction - واکنش زنجیره پلیمراز معکوس رونویسیGlial fibrillary acidic protein - پروتئین اسیدی فیبریلاسیون گلایالPatch-clamp - پچ گیرGABAA - گاباGABA - گاباGABAA receptor - گیرنده GABAA یا گیرنده گابا
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Although astrocytes express γ-aminobutyric acid subtype-A (GABAA) receptors in the mature brain, GABAA receptor expression in a cultivation state remains controversial. In this study, we investigated the alteration of astrocytic GABAA receptor expression in in vitro and in vivo studies to elucidate the relevance of astrocytic activation to reductions of astrocytic GABAA receptors. The GABA-evoked Clâ current (GABAA response) in cultured astrocytes was determined by recording in the whole-cell mode using a conventional patch-clamp technique under voltage-clamp conditions. The respective amplitudes of GABAA responses on days in vitro 1, 3-5, 7-10, and 12-15 were 1019 ± 97, 512 ± 76, 84 ± 21, and 22 ± 9 pA, respectively, suggesting that the GABAA response subsequently diminished with in vitro aging. In immunohistochemical and biochemical analyses, the expression of GABAA receptor β-subunit decreased, whereas expressions of glial fibrillary acidic protein (GFAP) and S100B, hallmarks of astrocytic activation, increased dramatically in the cultured astrocytes with in vitro aging. With the use of [3H]SR95531, a GABAA-specific ligand, at 24 h after transient focal ischemia, binding was significantly reduced in the astrocytic fractions without affecting the synaptosomal fractions, and decreases in the mRNA expression level of GABAA receptor β-subunits were concurrently observed. Interestingly, the loss of GABAA response in cultured astrocytes was mitigated by co-culturing with neurons or treatments with monoclonal S100B antibodies. These results indicate that astrocytic GABAA receptors are reduced with in vitro aging and cerebral ischemia, presumably through the overproduction of S100B in activated astrocytes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1089, Issue 1, 17 May 2006, Pages 79-91
Journal: Brain Research - Volume 1089, Issue 1, 17 May 2006, Pages 79-91
نویسندگان
Narito Tateishi, Taiji Shimoda, Jun-ichiro Manako, Seishi Katsumata, Rika Shinagawa, Hiroyuki Ohno,