Embedding matrix for simultaneous processing of multiple histological samples

► An improved method for embedding tissue in gelatin-agarose matrix is described. ► A cross-linking reagent rapidly solidifies the matrix and binds it to the tissue. ► The procedure allows simultaneous processing of multiple samples. ► A contrast reagent facilitates 3-dimensional reconstructions from digital images. ► The method is shown for multiple rodent brains, and fragmented human brain tissue.