کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4340536 | 1295801 | 2008 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Neurochemistry of the afferents to the rat cochlear root nucleus: Possible synaptic modulation of the acoustic startle
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کلمات کلیدی
DAB3,3′ diaminobenzidine tetrahydrochlorideVNTBVGLUTTH-irVGLUT1VGATGlyRGluRAVCNBDAN-methyl-d-aspartateNMDAABCPBSPNCASRPFCRT-PCRPPIThree-dimensionalCRNiGluR - Iglutrapezoid body - بدن ترسناکReceptor expression - بیان گیرندهbiotinylated dextran amine - بیوترنیته دیستران آمینtyrosine hydroxylase - تیروزین هیدروکسیلازtyrosine hydroxylase immunoreactive - تیروستین هیدروکسیلاز ایمنوراواکسیونvesicular GABA transporter - حامل ویسکوزر GABAacoustic startle reflex - رفلکس شگفت انگیز صوتیprefrontal cortex - قشر prefrontallocus coeruleus - لوکوس سیرولئوسavidin–biotin–peroxidase complex - مجتمع آویدین-بیوتین پراکسیدازPrepulse inhibition - مهار پیش قاعدگیlight microscopy - میکروسکوپ نوریConfocal microscopy - میکروسکوپ کانفوکالcaudal pontine reticular nucleus - هسته رتیکولین پونتین کادالventral nucleus of the trapezoid body - هسته شکمی از بدن استپزوئیدanteroventral cochlear nucleus - هسته کچلیون anteroventralreverse transcription-polymerase chain reaction - واکنش زنجیره ای رونویسی-پلیمراز معکوسNeurotransmitter receptors - گیرنده های نوروترانسمیترGlutamate receptor - گیرنده گلوماتglycine receptor - گیرنده گلیسینionotropic glutamate receptor - گیرنده ی گلوتامات ionotropic
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Neurochemistry of the afferents to the rat cochlear root nucleus: Possible synaptic modulation of the acoustic startle Neurochemistry of the afferents to the rat cochlear root nucleus: Possible synaptic modulation of the acoustic startle](/preview/png/4340536.png)
چکیده انگلیسی
Afferents to the primary startle circuit are essential for the elicitation and modulation of the acoustic startle reflex (ASR). In the rat, cochlear root neurons (CRNs) comprise the first component of the acoustic startle circuit and play a crucial role in mediating the ASR. Nevertheless, the neurochemical pattern of their afferents remains unclear. To determine the distribution of excitatory and inhibitory inputs, we used confocal microscopy to analyze the immunostaining for vesicular glutamate and GABA transporter proteins (VGLUT1 and VGAT) on retrogradely labeled CRNs. We also used reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry to detect and localize specific neurotransmitter receptor subunits in the cochlear root. Our results show differential distributions of VGLUT1- and VGAT-immunoreactive endings around cell bodies and dendrites. The RT-PCR data showed a positive band for several ionotropic glutamate receptor subunits, M1-M5 muscarinic receptor subtypes, the glycine receptor α1 subunit (GlyRα1), GABAA, GABAB, and subunits of α2 and β-noradrenergic receptors. By immunohistochemistry, we confirmed that CRN cell bodies exhibit positive immunoreaction for the glutamate receptor (GluR) 3 and NR1 GluR subunits. Cell bodies and dendrites were also positive for M2 and M4, and GlyRα1. Other subunits, such as GluR1 and GluR4 of the AMPA GluRs, were observed in glial cells neighboring unlabeled CRN cell bodies. We further confirmed the existence of noradrenergic afferents onto CRNs from the locus coeruleus by combining tyrosine hydroxylase immunohistochemistry and tract-tracing experiments. Our results provide valuable information toward understanding how CRNs might integrate excitatory and inhibitory inputs, and hence how they could elicit and modulate the ASR.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 154, Issue 1, 12 June 2008, Pages 51-64
Journal: Neuroscience - Volume 154, Issue 1, 12 June 2008, Pages 51-64
نویسندگان
R. Gómez-Nieto, J.A.C. Horta-Junior, O. Castellano, M.J. Herrero-Turrión, M.E. Rubio, D.E. López,