کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4341047 | 1295821 | 2009 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Neuronal enriched endosomal protein of 21 kDa colocalizes with glutamate receptor subunit GLUR2/3 at the postsynaptic membrane
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
glutamate receptor interacting protein 1NGSPSDTBSTPAGAMPARBSA - BSAbovine serum albumin - آلبومین سرم گاوEndosome - اندوسومsodium phosphate buffer - بافر فسفات سدیمpostsynaptic density - تراکم PostinapticDIV - دیوdays in vitro - روز in vitronormal goat serum - سرم طبیعی بزTrafficking - قاچاقElectron microscopy - میکروسکوپ الکترونیNeuron - نورونSynapse - همایه یا سیناپسGRIP - گریپphosphate-activated glutaminase - گلوتامیناز فعال فسفاتAMPA receptor - گیرنده AMPAAMPA receptors - گیرنده های AMPA
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Neuronal enriched endosomal protein of 21 kDa colocalizes with glutamate receptor subunit GLUR2/3 at the postsynaptic membrane Neuronal enriched endosomal protein of 21 kDa colocalizes with glutamate receptor subunit GLUR2/3 at the postsynaptic membrane](/preview/png/4341047.png)
چکیده انگلیسی
Functional evidence suggests that neuronal enriched endosomal protein of 21 kDa (NEEP21) takes part in facilitating transport of AMPA receptors (AMPAR) in the synapse. To explore the anatomical basis for a role in this synaptic trafficking, we investigated the ultrastructural localization of NEEP21 in rodent brain. Using immunogold electron microscopy, we show that NEEP21 is colocalized with the AMPAR subunits GluR2/3 in postsynaptic spines. Quantitative analysis of gold particle distribution along an axis perpendicular to the postsynaptic specialization indicated that NEEP21 occurs in the postsynaptic membrane but also in the interior of the spines. NEEP21 positive endosomes/multivesicular bodies were found throughout cell bodies and dendrites. In light microscopical preparations, the NEEP21 antibody produced a labeling pattern in the neocortex, hippocampus and cerebellum that mimicked that of GluR2/3 and not that of GluR1 or 4. Our findings are consistent with a role for NEEP21 in facilitating vesicular transport of GluR2 between intracellular compartments and the postsynaptic plasma membrane.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 158, Issue 1, 12 January 2009, Pages 96-104
Journal: Neuroscience - Volume 158, Issue 1, 12 January 2009, Pages 96-104
نویسندگان
J.K. Utvik, C. Haglerød, M.N.I. Mylonakou, T. Holen, M. Kropf, H. Hirling, Ã. Skare, P. Laake, O.P. Ottersen, F.-M.S. Haug, S. Davanger,