کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4341094 | 1295822 | 2007 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Different roles of nitric oxide synthase-1 and -2 between herpetic and postherpetic allodynia in mice
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کلمات کلیدی
Tris-buffered saline containing Tweenprimary sensory nerveNADPHGAPDHS-Methylisothiourea sulfateqRT-PCRNOSSMTTBS-TPBS7-NIHSV-17-nitroindazole - 7-نیتریدازولHerpes zoster - زونا، هرپس زوسترSacral - ساکرالdorsal horn - شاخ پشتیlumbar - لامبار، ناحیهٔ کمرPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریpostherpetic neuralgia - نورالژی پسرشپتیکNitric oxide - نیتریک اکسیدnitric oxide synthase - نیتریک اکسید سنتازnicotinamide adenine dinucleotide phosphate - نیکوتین آمید adenine dinucleotide phosphatequantitative reverse transcription-polymerase chain reaction - واکنش زنجیره ای رونویسی معکوس و پلیمریزا معکوسHerpes simplex virus type-1 - ویروس هرپس سیمپلکس نوع 1glyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We investigated using the mice role of nitric oxide synthase (NOS) in the spinal dorsal horn in herpetic and postherpetic pain, especially allodynia, which was induced by transdermal inoculation of the hind paw with herpes simplex virus type-1 (HSV-1). The virus inoculation induced NOS2 expression in the lumbar dorsal horn of mice with herpetic allodynia, but not postherpetic allodynia. There were no substantial alternations in the expression level of NOS1 at the herpetic and postherpetic stages. Herpetic allodynia was significantly inhibited by i.p. administration of the selective NOS2 inhibitor S-methylisothiourea, but not the selective NOS1 inhibitor 7-nitroindazole. NOS2 expression was observed around HSV-1 antigen-immunoreactive cells. On the other hand, postherpetic allodynia was significantly inhibited by i.p. administration of 7-nitroindazole, but not S-methylisothiourea. The activity of reduced nicotinamide adenine dinucleotide phosphate diaphorase, an index of NOS1 activity, significantly increased in the laminae I and II of the lumbar dorsal horn of mice with postherpetic allodynia, but not mice without postherpetic allodynia. The expression level of NOS1 mRNA in the dorsal root ganglia was similar between mice with and without postherpetic allodynia. The results suggest that herpetic and postherpetic allodynia is mediated by nitric oxide in the dorsal horn and that NOS2 and NOS1 are responsible for herpetic and postherpetic allodynia, respectively. It may be worth testing the effects of NOS2 and NOS1 inhibitors on herpetic pain and postherpetic neuralgia in human subjects, respectively.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 150, Issue 2, 5 December 2007, Pages 459-466
Journal: Neuroscience - Volume 150, Issue 2, 5 December 2007, Pages 459-466
نویسندگان
A. Sasaki, T. Mabuchi, K. Serizawa, I. Takasaki, T. Andoh, K. Shiraki, S. Ito, Y. Kuraishi,