کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4341894 | 1295849 | 2006 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Photobiomodulation partially rescues visual cortical neurons from cyanide-induced apoptosis
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کلمات کلیدی
DABPBSNIRCM-H2DCFDAssDNAHRP3,3′-diaminobenzidine - 3،3'-diaminobenzidineSingle-stranded DNA - DNA تک رشته ایKCN - IP هاROS - ROSRoom temperature - دمای اتاقlight-emitting diode - دیود نورافشانLED - رهبریPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریNear-infrared - نزدیک مادون قرمزNear-infrared light - نور مادون قرمز نزدیکpotassium cyanide - پتاسیم سیانیدHorseradish peroxidase - پراکسیداز هوررادیشCaspase-3 - کاسپاز ۳ Reactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Near-infrared light via light-emitting diode treatment has documented therapeutic effects on neurons functionally inactivated by tetrodotoxin or methanol intoxication. Light-emitting diode pretreatment also reduced potassium cyanide-induced cell death, but the mode of death via the apoptotic or necrotic pathway was unclear. The current study tested our hypothesis that light-emitting diode rescues neurons from apoptotic cell death. Primary neuronal cultures from postnatal rat visual cortex were pretreated with light-emitting diode for 10 min at a total energy density of 30 J/cm2 before exposing to potassium cyanide for 28 h. With 100 or 300μM potassium cyanide, neurons died mainly via the apoptotic pathway, as confirmed by electron microscopy, Hoechst 33258, single-stranded DNA, Bax, and active caspase-3. In the presence of caspase inhibitor I, the percentage of apoptotic cells in 300μM potassium cyanide was significantly decreased. Light-emitting diode pretreatment reduced apoptosis from 36% to 17.9% (100μM potassium cyanide) and from 58.9% to 39.6% (300μM potassium cyanide), representing a 50.3% and 32.8% reduction, respectively. Light-emitting diode pretreatment significantly decreased the expression of caspase-3 elicited by potassium cyanide. It also reversed the potassium cyanide-induced increased expression of Bax and decreased expression of Bcl-2 to control levels. Moreover, light-emitting diode decreased the intensity of 5-(and -6) chloromethy-2â², 7-dichlorodihydrofluorescein diacetate acetyl ester, a marker of reactive oxygen species, in neurons exposed to 300μM potassium cyanide. These results indicate that light-emitting diode pretreatment partially protects neurons against cyanide-induced caspase-mediated apoptosis, most likely by decreasing reactive oxygen species production, down-regulating pro-apoptotic proteins and activating anti-apoptotic proteins, as well as increasing energy metabolism in neurons as reported previously.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 139, Issue 2, 2006, Pages 639-649
Journal: Neuroscience - Volume 139, Issue 2, 2006, Pages 639-649
نویسندگان
H.L. Liang, H.T. Whelan, J.T. Eells, H. Meng, E. Buchmann, A. Lerch-Gaggl, M. Wong-Riley,