کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4343895 1615143 2013 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Strongly alkaline pH avoidance mediated by ASH sensory neurons in C. elegans
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Strongly alkaline pH avoidance mediated by ASH sensory neurons in C. elegans
چکیده انگلیسی


• pH higher than 10.5 is a noxious stimulus to C. elegans.
• ASH nociceptive neurons serve as sensors for strongly alkaline pH.
• OSM-9/OCR-2 TRPV functions as a channel for Ca2+ influx into ASH.
• High pH may also be sensed by nociceptors in mammals.

High pH is a noxious stimulus to animals, and their ability to avoid dangerously alkaline pH is critical for survival. However, the means by which they sense high pH has not been determined. The nematode Caenorhabditis elegans (C. elegans) avoids environmental pH above 10.5. In contrast, C. elegans mutants with structurally, developmentally, and/or functionally abnormal sensory cilia fail to avoid high pH, suggesting that sensory neurons in the cilia participate in sensing. Genetic rescue of the mutants indicates that ASH polymodal sensory neurons play a vital role in the process. Consistently, specific laser ablation of ASH neurons made animals insensitive to high pH. Furthermore, avoidance assays of other mutants also indicated that transient receptor potential vanilloid type (TRPV) ion channels encoded by osm-9 and ocr-2 are involved in sensing. Indeed, genetic rescue of osm-9 mutants by specifically expressing OSM-9 in ASH showed that TRPV channels play an essential role in sensing of high pH. Ca2+ imaging in vivo also revealed that ASH neurons were activated by high pH stimulation, but ASH of osm-9 or ocr-2 mutants were not. These results demonstrate that in C. elegans, high pH is sensed by ASH nociceptors through opening of OSM-9/OCR-2 TRPV channels.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 555, 25 October 2013, Pages 248–252
نویسندگان
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