Regionally varying F-actin network in the apical cytoplasm of ependymocytes

F-actin participates in morphogenetic cell-shape changes and helps maintain cellular integrity. Actin-like proteins have been detected in the ependymocytes of the cerebral ventricles, but the distribution of F-actin along the ventricular system has not been studied. We observed a highly ordered and regionally varying F-actin network in the apical cytoplasm of the ependyma in the ventricular system of rats using fluorescein isothiocyanate-conjugated phalloidin. Dense F-actin bundles spanned the entire circumference of the central canal of the spinal cord and formed a characteristic ring-like network in the apical region. The apical F-actin layer was widest in the lower cervical canal, and narrower in the upper thoracic canal. However, in the lower part of the filum terminale, the apical F-actin bundles became sparser and even disappeared. The apical F-actin layer differs significantly between the ventral and dorsal aspects above the medulla oblongata. This suggests that the regionally varying distribution of F-actin reflects the diverse local demands of the ependymocytes for cellular integrity and adhesive activity against external forces.