کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4366729 1616592 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of lactobacilli with inhibitory effect on biofilm formation by pathogenic bacteria on stainless steel surfaces
ترجمه فارسی عنوان
شناسایی لاکتوباسیل با اثر مهاری بر تشکیل بیوفیلم توسط باکتری های بیماریزا بر روی سطوح از جنس فولاد ضد زنگ
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
چکیده انگلیسی


• Richness of atypical ecological niches as providers of strains with potential antagonism
• Anti-staphylococcal adhesion of Lactobacillus pentosus LB3F2 to stainless steel slides
• Production of BLIS with antagonism against Gram negative bacteria
• Evidences on stimulation of metabolic activity of Lactobacillus pentosus

Two hundred and thirty individual clones of microorganisms were recovered from milk tanks and milking machine surfaces at two distinct farms (Bejaja City, Algeria). Of these clones, 130 were identified as lactic acid bacteria (LAB). In addition Escherichia coli, Salmonella, Staphylococcus aureus and Pseudomonas aeruginosa species were identified in the remaining 100 isolates—spoilage isolate. These isolates were assayed for ability to form biofilms. S. aureus, Lactobacillus brevis strains LB1F2, LB14F1 and LB15F1, and Lactobacillus pentosus strains LB2F2 and LB3F2 were identified as the best biofilm formers. Besides, these LAB isolates were able to produce proteinaceous substances with antagonism against the aforementioned spoilage isolates, when grown in MRS or TSB-YE media. During the screening, L. pentosus LB3F2 exhibited the highest antibacterial activity when grown in TSB-YE medium at 30 °C. Additionally, L. pentosus LB3F2 was able to strongly hamper the adhesion of S. aureus SA3 on abiotic surfaces as polystyrene and stainless steel slides. LAB isolates did not show any hemolytic activity and all of them were sensitive to different families of antibiotic tested. It should be pointed out that LB3F2 isolate was not cytotoxic on the intestinal cells but could stimulate their metabolic activity. This report unveiled the potential of LB1F2, LB14F1, LB15F1, LB2F2, and LB3F2 isolates to be used as natural barrier or competitive exclusion organism in the food processing sector as well as a positive biofilm forming bacteria.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Food Microbiology - Volume 191, 17 November 2014, Pages 116–124
نویسندگان
, , , , , , , ,