کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4492913 1623304 2013 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Molecular characterization of a cucumber mosaic cucumovirus isolated from lettuce in Egypt
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
Molecular characterization of a cucumber mosaic cucumovirus isolated from lettuce in Egypt
چکیده انگلیسی

Cucumber mosaic cucumovirus (CMV) was isolated from lettuce plants (Lactuca sativa) showing virus like symptoms. Isolation was performed depending on specific polyclonal antibodies and Chenopodium quinoa as a local lesion host. Virus was purified from 200 gm of virus-infected Nicotiana tabacum cv. White Burley leaves giving A260/280 ratio of 1.21 and a yield of 1.7 mg. Purified virus preparation was used for rabbit immunization to produce specific polyclonal antibodies. IgGs were purified and evaluated by indirect enzyme linked immunosorbant assay (I-ELISA) to determine the dilution end point which found to be 1:512. Electron micrographs showed spherical virus particles of about 30 nm in diameter. Virus coat protein (CP) molecular weight was determined using sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE), giving a single band of 25 kDa within resolving gel. Immunocapture-reverse transcriptase-polymerase chain reaction (IC-RT-PCR) was used for the amplification of CMV coat protein gene (cp), the appearance of 657 bp bands confirmed the expected size of such gene. Comparing virus cp gene sequence with the sequences of seven overseas isolates confirmed that the under study isolate was related to the CMV subgroup I.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Annals of Agricultural Sciences - Volume 58, Issue 1, June 2013, Pages 105–109
نویسندگان
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