کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4505510 1624295 2017 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification and characterization of Alternaria alternata causing leaf spot of olive tree (Olea europaea) in Turkey
ترجمه فارسی عنوان
شناسائی و تعیین خصوصیات و Alternaria alternata باعث نقطه برگ درخت زیتون (گونه Olea europaea) در ترکیه
کلمات کلیدی
و Alternaria alternata؛ متعارف، PCR، Olea europaea است. نقطه برگ زیتون؛ Real-time PCR
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم زراعت و اصلاح نباتات
چکیده انگلیسی


• This is the first report of the leaf spot of olive tree in Turkey.
• A. alternata was identified as the causal agent of the disease in Turkey.
• The results will provide effective management of this disease in case of the outbreak.

During 2015, 2016 surveys, unusual leaf spot symptoms were observed on Gemlik and Ayvalık varieties of olive trees in various orchards in Antalya province in the western Mediterranean region of Turkey. The symptoms appeared as powdery or mycelium-like spots with diameters ranging from 3 to 7 mm on the leaves and stems. The symptoms were observed only in the inner portion of the olive leaves or branches of the older trees, but found on every portion of the leaves of younger trees. The fungus was successfully isolated from the leaves and cultured in vitro. The colonies were white-grey airy mycelium with the mixture of green; and light dark brown inner zones which change to light dark brown; green in the later period of growth. Dark brown conidia in chains ranged from 7 to 45.9 μm in length were observed under microscopic examination. Molecularly, the isolates were subjected to both conventional and Real-time Polymerase Chain Reaction (PCR) using AaltFor/AaltRev, AAF2/AAR3 and ITS2/ITS4 primers which produced approximately 184, 346 and 560 bp, respectively for conventional PCR. The isolates also tested positive with SYBR Green dye and AaltFor/AaltRev primer pair in the Real-time PCR test. The isolates showed high similarity based on the ITS region sequence and the beta tubulin gene among the monophyletic clade of the A. alternata in the inner group and that of those of GenBank, ranged from 95 to 100% for all the three primers tested. The prevalence of the disease was estimated between 40 and 80% in three of the orchards. Pathogenicity of the fungus also produced a positive result on the two-year-old Gemlik and Ayvalık olive seedling varieties, confirming their pathogenic status. This is the first report implicating A. alternata as the causal agent of leaf spot disease of olive trees in Turkey.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Crop Protection - Volume 92, February 2017, Pages 79–88
نویسندگان
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