Green synthesis and characterization of silver nanoparticles using Boerhaavia diffusa plant extract and their anti bacterial activity

• First report on the biosynthesis of Ag NPs using Boerhaavia diffusa plant extract.
• Act as a good antibacterial agent against various fish bacterial pathogens.
• Ag NPs were characterized with UV–Vis, XRD, SEM and TEM.
• Ag NPs were spherical shape with average size of about 25 nm.

Green synthesis of silver nanoparticles was carried out using Boerhaavia diffusa plant extract as a reducing agent. The biosynthesized AgNPs were characterized by SEM-EDAX, XRD, TEM, UV–Vis, and FT-IR spectroscopy techniques. UV–Vis spectroscopy of prepared silver colloidal solution showed absorption maxima at 418 nm. XRD and TEM analysis revealed that AgNPs are face-centered, cubic structure being spherical in shape with an average particle size of 25 nm. The AgNPs were tested for antibacterial activity against three fish bacterial pathogens viz., Aeromonas hydrophila, Pseudomonas fluorescens and Flavobacterium branchiophilum and they demonstrated highest sensitivity toward F. branchiophilum when compared to other two bacterial pathogens.

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