کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4557553 | 1628215 | 2016 | 5 صفحه PDF | دانلود رایگان |
• PCR showed Ceratonova DNA in specimens of Manayunkia speciosa.
• rDNA and EF2 molecular data show these parasites are genetically distinct.
• ITS barcoding sequences suggest parasites are undescribed species.
• This is the first report of Ceratonova outside the Pacific Northwest, USA.
We used PCR to screen pooled individuals of Manayunkia speciosa from western Lake Erie, Michigan, USA for myxosporean parasites. Amplicons from positive PCRs were sequenced and showed a Ceratonova species in an estimated 1.1% (95% CI = 0.46%, 1.8%) of M. speciosa individuals. We sequenced 18S, ITS1, 5.8S, ITS2 and most of the 28S rDNA regions of this Ceratonova sp., and part of the protein-coding EF2 gene. Phylogenetic analyses of ribosomal and EF2 sequences showed the Lake Erie Ceratonova sp. is most similar to, but genetically distinct from, Ceratonova shasta. Marked interspecific polymorphism in all genes examined, including the ITS barcoding genes, along with geographic location suggests this is an undescribed Ceratonova species. COI sequences showed M. speciosa individuals in Michigan and California are the same species. These findings represent a third parasite in the genus Ceratonova potentially hosted by M. speciosa.
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Journal: Journal of Invertebrate Pathology - Volume 137, June 2016, Pages 49–53