Desiccation survival of Acinetobacter spp. in infant formula

• Acinetobacter spp. are able to maintain their viability in desiccated infant formula for 2 years.
• Acinetobacter spp. are able to efficiently recover after reconstitution despite long-term desiccation.
• E. cloacae and E. hormaechei were more sensitive to desiccation than the Acinetobacter strains.
• The persistence of Acinetobacter spp. strains in desiccated powdered infant formula supports the FAO-WHO designation of this organism as a risk to neonatal health.

Acinetobacter spp. are included under category B in the FAO-WHO list of organisms of concern for neonatal health following the consumption of powdered infant formula. However, the ability of Acinetobacter spp. to maintain their viability in desiccated infant formula over a storage period consistent with the shelf-life of commercially available powdered infant formula (2 years) has not been demonstrated. In this study, 9 clinical and food isolates of Acinetobacter baumannii, A. calcoaceticus, and Acinetobacter genomosp. 3 were desiccated in infant formula and then reconstituted at designated time points. Bacterial viability was followed for a maximum period of 24 months or until the strain became undetectable (<5 × 102 cfu/ml). For comparative purposes, one Enterobacter hormaechei and two Enterobacter cloacae strains were also monitored for their desiccation survival. The seven clinical and food strains remained cultivable for the whole duration of the study and showed biphasic survival curves. The initial drop in viable count was up to 3.5 log10 cfu/ml within 18 h of desiccation exposure. By the end of the study, the reduction in viability was between 3.6 and 4.8 log10 cfu/ml. In contrast the A. baumanii and A. calcoaceticus species type strains only persisted for 6 and 9 months, respectively, possibly due to laboratory adaptation. The E. cloacae and E. hormaechei strains were undetectable after 12 and 17 months, respectively. The persistence of Acinetobacter spp. strains in desiccated powdered infant formula, supports the FAO-WHO designation of this organism as a risk to neonatal health.