کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5504074 | 1535860 | 2017 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Melatonin modulates monochromatic light-induced melatonin receptor expression in the hypothalamus of chicks
ترجمه فارسی عنوان
ملاتونین مولکول بیان یک گیرنده ملاتونین القا شده توسط نور را در هیپوتالاموس جوجه ها
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کلمات کلیدی
نور تک رنگ ملاتونین، گیرنده ملاتونین، هیپوتالاموس، جوجه،
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
To study the mechanism of the effect of monochromatic light on physiological function in chicken, a total of 192 newly hatched chicks were randomly divided into intact, sham-operated and pinealectomy groups then exposed to white light (WL), red light (RL), green light (GL) and blue light (BL) using a light-emitting diode (LED) system for two weeks. At P14, the hypothalami were immediately collected for immunohistochemical staining of melatonin receptor subtypes (Mel1a and Mel1b) and detection of Mel1a and Mel1b expressions using RT-PCR and western blot. Immunohistochemical staining of the hypothalamus showed that the Mel1a-ir cells were distributed in the preoptic area (POA), nucleus preopticus periventricularis (POP) and suprachiasmatic nuclei (SCN), and the Mel1b-ir cells were presented in the POA and SCN. Analysis of RT-PCR and western blot showed that the mRNA and protein levels of Mel1a and Mel1b in the hypothalamus of chick exposed to GL were increased by 10.7-29.3%, 9.18-35.9% and 8.97-27.3% compared to those in the chicks exposed to WL (PÂ =Â 0.029-0.002), RL (PÂ =Â 0.027-0.001) and BL (PÂ =Â 0.038-0.007) in the intact group, respectively. After pinealectomy, however, these parameters decreased and there were no significant differences among the WL, RL, GL and BL groups. These findings suggested that melatonin plays a critical role in GL illumination-enhanced Mel1a and Mel1b expressions in the hypothalamus of chicks.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Acta Histochemica - Volume 119, Issue 7, September 2017, Pages 733-739
Journal: Acta Histochemica - Volume 119, Issue 7, September 2017, Pages 733-739
نویسندگان
Liwei Zhang, Funing Chen, Jing Cao, Yulan Dong, Zixu Wang, Yaoxing Chen,