کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5508278 | 1537682 | 2018 | 23 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Phospholipid composition of packed red blood cells and that of extracellular vesicles show a high resemblance and stability during storage
ترجمه فارسی عنوان
ترکیب فسفولیپید از گلبول های قرمز بسته بندی شده و حامل های خارج سلولی، مشابه بودن و پایداری در حین ذخیره سازی
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کلمات کلیدی
گلبول های قرمز ذخیره شده، ضایعه ذخیره سازی، حامل های خارج سلولی، فسفولیپید ها، طیف سنجی جرمی،
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Red blood cells (RBCs) are stored up to 35-42 days at 2-6 °C in blood banks. During storage, the RBC membrane is challenged by energy depletion, decreasing pH, altered cation homeostasis, and oxidative stress, leading to several biochemical and morphological changes in RBCs and to shedding of extracellular vesicles (EVs) into the storage medium. These changes are collectively known as RBC storage lesions. EVs accumulate in stored RBC concentrates and are, thus, transfused into patients. The potency of EVs as bioactive effectors is largely acknowledged, and EVs in RBC concentrates are suspected to mediate some adverse effects of transfusion. Several studies have shown accumulation of lipid raft-associated proteins in RBC EVs during storage, whereas a comprehensive phospholipidomic study on RBCs and corresponding EVs during the clinical storage period is lacking. Our mass spectrometric and chromatographic study shows that RBCs maintain their major phospholipid (PL) content well during storage despite abundant vesiculation. The phospholipidomes were largely similar between RBCs and EVs. No accumulation of raft lipids in EVs was seen, suggesting that the primary mechanism of RBC vesiculation during storage might not be raft -based. Nonetheless, a slight tendency of EV PLs for shorter acyl chains was observed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1863, Issue 1, January 2018, Pages 1-8
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1863, Issue 1, January 2018, Pages 1-8
نویسندگان
Eva Laurén, Feven Tigistu-Sahle, Sami Valkonen, Melissa Westberg, Anne Valkeajärvi, Juha Eronen, Pia Siljander, Ville Pettilä, Reijo Käkelä, Saara Laitinen, Erja Kerkelä,