کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5510951 1539374 2017 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Polμ deficiency induces moderate shortening of P53−/− mouse lifespan and modifies tumor spectrum
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Polμ deficiency induces moderate shortening of P53−/− mouse lifespan and modifies tumor spectrum
چکیده انگلیسی
Non-homologous end joining (NHEJ) is the main mechanism for double strand break (DSB) DNA repair. The error-prone DNA polymerase mu (Polμ) is involved in immunoglobulin variable region rearrangement and in general, NHEJ in non-lymphoid cells. Deletion of NHEJ factors in P53−/− mice, which are highly prone to development of T cell lymphoma, generally increases cancer incidence and shifts the tumor spectrum towards aggressive pro-B lymphoma. In contrast, Polμ deletion increased sarcoma incidence, proportionally reducing pro-B lymphoma development on the P53-deficient background. Array comparative genomic hybridization (aCGH) analyses showed DNA copy number alterations in both P53−/− and Polμ−/−P53−/− lymphomas. Our results also indicate that the increase in sarcoma incidence in Polμ−/−P53−/− mice could be associated with Cdk4 and Kub3 amplification and overexpression. These results identify a role for Polμ in the prevention of sarcomagenesis on a murine P53-deficient background, in contrast to most other NHEJ factors.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: DNA Repair - Volume 54, June 2017, Pages 40-45
نویسندگان
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