کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5511093 1539462 2017 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A new Drosophila octopamine receptor responds to serotonin
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش حشره شناسی
پیش نمایش صفحه اول مقاله
A new Drosophila octopamine receptor responds to serotonin
چکیده انگلیسی


- An orphan gene CG18208 from the fruit fly, Drosophila melanogaster was characterized.
- CG18208 encodes two transcripts by alternative splicing to generate DmOctα2R-L and DmOctα2R-S.
- Octopamine, tyramine and serotonin can activate DmOctα2R to inhibit cAMP production.
- Adrenergic and serotoninergic agonists and antagonists are effective on DmOctα2R.

As the counterparts of the vertebrate adrenergic transmitters, octopamine and tyramine are important physiological regulators in invertebrates. They control and modulate many physiological and behavioral functions in insects. In this study, we reported the pharmacological properties of a new α2-adrenergic-like octopamine receptor (CG18208) from Drosophila melanogaster, named DmOctα2R. This new receptor gene encodes two transcripts by alternative splicing. The long isoform DmOctα2R-L differs from the short isoform DmOctα2R-S by the presence of an additional 29 amino acids within the third intracellular loop. When heterologously expressed in mammalian cell lines, both receptors were activated by octopamine, tyramine, epinephrine and norepinephrine, resulting in the inhibition of cAMP production in a dose-dependent manner. The long form is more sensitive to the above ligands than the short form. The adrenergic agonists naphazoline, tolazoline and clonidine can stimulate DmOctα2R as full agonists. Surprisingly, serotonin and serotoninergic agonists can also activate DmOctα2R. Several tested adrenergic antagonists and serotonin antagonists blocked the action of octopamine or serotonin on DmOctα2R. The data presented here reported an adrenergic-like G protein-coupled receptor activated by serotonin, suggesting that the neurotransmission and neuromodulation in the nervous system could be more complex than previously thought.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Insect Biochemistry and Molecular Biology - Volume 90, November 2017, Pages 61-70
نویسندگان
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