کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5511591 | 1540213 | 2017 | 13 صفحه PDF | دانلود رایگان |
Chitosan (CS), an amino polysaccharide has fascinating scientific applications due to its many flexible properties. The advantages of Chitosan tend to increase when it was modified. Thus, in the present research work, to improve the properties of chitosan, it was converted into chitosan nanoparticles (CS-NPs) through the ionic gelation method using sodium tripoyphosphate (TPP) and sodium hexametaphosphate (SHMP) as a crosslinker. The size optimization was done by varying the parameters such as crosslinker concentration, agitation method and rate, agitation time, temperature and drying method. The prepared samples were characterized using FTIR, TGA, XRD, SEM, TEM and DLS. Also the prepared CS-NPs with TPP and SHMP had been evaluated in vitro for determining its hemocompatibility, biodegradability, serum stability, cytotoxicity and cell viability. The results showed the significant participation of all the parameters in obtaining the nanoparticles in 20-30Â nm and 5-10Â nm for CS-NPs-TPP air dried and freeze dried samples and around 60-80Â nm and 20-30Â nm for CS-NPs-SHMP air dried and freeze dried samples. The in vitro biological studies revealed that the nanoparticles are non-toxic with a good degree of biodegradability, blood compatibility and stability.
Journal: International Journal of Biological Macromolecules - Volume 104, Part B, November 2017, Pages 1794-1806