Dithiocarbamate to modify magnetic graphene oxide nanocomposite (Fe3O4-GO): A new strategy for covalent enzyme (lipase) immobilization to fabrication a new nanobiocatalyst for enzymatic hydrolysis of PNPD

- Dithiocarbamate to modify magnetic graphene oxide nanocomposite (Fe3O4-GO) for covalent enzyme (lipase) immobilization.
- Post-synthesis of dithiocarbamate on the surface of Fe3O4/GO-NH2 to Fe3O4/GO-NHCS2H for immobilization of lipase.
- Fabrication Fe3O4/GO-NHCS-Lip as a nanobiocatalyst for enzymatic hydrolysis of PNPD.

Immobilization of lipase was successfully achieved on the surface of magnetically separable Fe3O4/graphene oxide (GO) via a post-modification. This post modification was achieved in alternation to glutaraldehyde post-modification. The activity of immobilized lipase had not a significant loss in the activity while on the other hand, it is simply extractable (by keeping its major activity) from reaction crude by a magnet. Each step of immobilization was carefully monitored by characterization and all were successfully proved. SEM, TEM, XRD, EDX, and FTIR were used to characterize the support and immobilization process.

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