کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5513033 | 1540975 | 2017 | 33 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of synthetic ecdysteroid analogues as functional mimics of brassinosteroids in plant growth
ترجمه فارسی عنوان
تشخیص آنالوگهای سنتتیک اگزاستروئیدی به عنوان عملکردهای عملکردی براسینستروئید در رشد گیاه
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کلمات کلیدی
qRT–PCRbrassinazoleECDBRZNOE20-HydroxyecdysoneBRSDMSO - DMSOArabidopsis thaliana - آرابیدوپسیس تالیاناnuclear overhauser effect - اثر بیش از حد هسته ایhypocotyl elongation - انقباض هیپوکوتییلBrassinosteroid - براسین استروئیدBrassinolide - براسینولیدBrassinosteroids - برسینستروئید هاDimethyl sulfoxide - دیمتیل سولفواکسیدDAY - روزHydroxysteroid - هیدروکسیستروئیدquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Brassinosteroids (BRs) are plant steroidal hormones that play important roles in many stages of plant growth. Several plant species produce ecdysteroids, which are known as insect molting steroid hormones. In this study, we evaluated the biological activities of three hydroxysteroidal compounds, 20-hydroxyecdysone (ECD), 7,8-dihydro-8α-20-hydroxyecdysone (DHECD), and 7,8-dihydro-5α,8α-20-hydroxyecdysone (α-DHECD), and compared their activities with that of brassinolide (BL), the most potent BR. In rice, DHECD and α-DHECD enhanced the degree of lamina inclination, as do BRs. In Arabidopsis thaliana, DHECD and α-DHECD increased hypocotyl length in the wild-type, and also partially overcame the hypocotyl shortening in the wild-type caused by 0.3 μM brassinazole, a specific BR biosynthesis inhibitor. DHECD and α-DHECD partially reduced dwarfism in the BR-biosynthesis-deficient mutant det2. Treatment with DHECD or α-DHECD downregulated the expression of the BR biosynthesis genes DWF4 and CPD, which are generally, suppressed by BR, and upregulated the expression of TCH4 and SAUR-AC1, which are generally promoted by BR. However, their regulated activities were less effective than BL. Moreover, the 10â4 M DHECD and α-DHECD induced the accumulation of dephosphorylated BIL1/BZR1 that enhanced BR signaling as a master transcription factor. In contrast, ECD did not affect rice lamina bending, Arabidopsis hypocotyl elongation, the expression levels of BR-related genes and BIL1/BZR1 phosphorylation status. Based on these results, we hypothesize that both DHECD and α-DHECD have functional activities similar to those of BR.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Steroid Biochemistry and Molecular Biology - Volume 172, September 2017, Pages 1-8
Journal: The Journal of Steroid Biochemistry and Molecular Biology - Volume 172, September 2017, Pages 1-8
نویسندگان
Jutiporn Thussagunpanit, Kanapol Jutamanee, Sureeporn Homvisasevongsa, Apichart Suksamrarn, Ayumi Yamagami, Takeshi Nakano, Tadao Asami,