کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5513658 1541210 2017 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Affinity chromatography: A versatile technique for antibody purification
ترجمه فارسی عنوان
کروماتوگرافی وابستگی: تکنیک متنوع برای تصفیه آنتی بادی
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی


- Affinity-based antibody purification is reported to be the most efficient.
- Various choices for antibody purification exist with their own set of pros and cons.
- Antibody purification persists to be major bottleneck in antibody production.
- There is a continuous demand for simple, robust and efficient purification methods.

Antibodies continue to be extremely utilized entities in myriad applications including basic research, imaging, targeted delivery, chromatography, diagnostics, and therapeutics. At production stage, antibodies are generally present in complex matrices and most of their intended applications necessitate purification. Antibody purification has always been a major bottleneck in downstream processing of antibodies, due to the need of high quality products and associated high costs. Over the years, extensive research has focused on finding better purification methodologies to overcome this holdup. Among a plethora of different techniques, affinity chromatography is one of the most selective, rapid and easy method for antibody purification. This review aims to provide a detailed overview on affinity chromatography and the components involved in purification. An array of support matrices along with various classes of affinity ligands detailing their underlying working principles, together with the advantages and limitations of each system in purifying different types of antibodies, accompanying recent developments and important practical methodological considerations to optimize purification procedure are discussed.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 116, 1 March 2017, Pages 84-94
نویسندگان
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