کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5513661 1541210 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Electrochemical immunosensor for tumor necrosis factor-alpha detection in undiluted serum
ترجمه فارسی عنوان
ایمونوسنسور الکتروشیمیایی برای تشخیص نکروز تومور-آلفا در سرم ناشتا
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی

An immunosensor for the sensitive detection and estimation of tumor necrosis factor-alpha (TNF-α) in undiluted serum has been developed via an electrochemical enzyme-linked immunosorbent assay (ELISA) process. Electrochemical sensing was performed using a TNF-α specific monoclonal antibody modified self-assembled monolayer of dithiobis(succinimidyl propionate) on a comb-shaped gold electrode microarray. After anti-TNF-α antibody binding, unreacted active groups of DTSP were blocked using ethanol amine (EA) and nonspecific binding was prevented using phosphate buffer based starting block T20 (SB). Sensitive and disposable SB-EA-anti-TNF-α/DTSP/Au electrodes were exposed to solutions with different TNF-α concentrations for 20 min in undiluted serum. Conversion of 4-aminophenyl phosphate to 4-aminophenol and its electrochemical oxidation was utilized for indirect estimation of TNF-α. Results for SB-anti-TNF-α/DTSP/Au electrodes indicate that the sensors can be used for the sensitive estimation of TNF-α in undiluted serum in the range 500 pg/ml to 100 ng/ml with a detection limit of 60 pg/ml and sensitivity of 0.46 (ng/ml)−1. Negligible interference from serum and other biomarker proteins was observed. The described electrochemical ELISA is much faster than conventional ELISA and can be applied for sensing of a range of analytes in real patient samples.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 116, 1 March 2017, Pages 125-131
نویسندگان
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