کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5516147 1542308 2017 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Stop codon mutagenesis for homogenous expression of human papillomavirus L1 protein in Escherichia coli
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Stop codon mutagenesis for homogenous expression of human papillomavirus L1 protein in Escherichia coli
چکیده انگلیسی


- Only a TAG codon can completely terminate the translation of HPV31 L1 in E. coli.
- Translation read-through might reflect the structure and stability of RNA folding.
- HPV31 L1 (TAG) VLPs self-assemble into T = 7 icosahedral symmetry particles.
- HPV31 L1 (TAG) VLPs have excellent antigenicity and immunogenicity for HPV vaccines.

Human papillomavirus (HPV) is widely accepted to be the major causative pathogen of cervical cancer, warts, and other epithelial tumors. Virus infection and subsequent disease development can be prevented by vaccination with HPV vaccines derived from eukaryotic expression systems. Here, we report the soluble expression of the major capsid protein L1 of HPV31, a dominant carcinogenic HPV genotype, in Escherichia coli. HPV31 L1 protein and its elongated form (L1+) were observed in SDS-PAGE and CE-SDS analysis, generated by the native HPV31 L1 gene with a TAA stop codon. Replacing the TAA with TAG but not TGA could completely terminate protein translation. Mass spectrometry sequencing showed that L1+ comprised L1 with a C-terminal extension of 38 amino acids (aa). RNA folding analysis revealed that the unfaithful L1+ expression may result from translational read-through, as TAG is more stable and accessible than the other stop codons. The 38-aa elongated fragment perturbs self-assembly of HPV31 L1+, as shown in size and morphology analyses. By 3D cryo-electron microscopy structure determination, we show self-assembly of purified HPV31 L1 (TAG) VLPs into T = 7 icosahedral symmetry particles, resembling the native HPV virion. Finally, through additional characterization and antigenicity/immunogenicity assays, we verified that the E.coli-derived HPV31 VLPs are an ideal immunogen for HPV vaccine development. Our findings outline a codon optimization stratagem for protein expression and provide a method for the in-depth investigation of prokaryotic translation regulation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 133, May 2017, Pages 110-120
نویسندگان
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