Response and feedback of C mineralization to P availability driven by soil microorganisms

- P deficiency had negative effects on soil C storage with a higher respiratory C-loss.
- P availability influenced C mineralization through changes in bacterial community.
- Changes in microbial community and activity by glucose were sustained for at least 70 days.
- Glucose induced a stimulation of P-solubilizing bacteria in P-deficient soils.
- Labile C improved soil P availability by the roles of soil microorganisms.

Despite our current understanding of soil C and N interactions, less is known about the response and feedback of C mineralization to soil P availability driven by microorganisms. To better understand these interactions, soils with long-term P-sufficient NPK fertilization (available P: 13.4 mg kg−1) and P-deficient NK fertilization (available P: 0.96 mg kg−1) were incubated with and without glucose. CO2 emissions were monitored to characterize C mineralization during the incubation. The soil bacterial community structure, quantity and metabolic activity were evaluated using high-throughput sequencing, qPCR and microcalorimetric dynamics. Compared with P-sufficient soils, P-deficient soils had significantly lower basal respiration, but a significantly higher net mineralization of added glucose, probably due to higher energy cost of soil microorganisms. Glucose addition promoted microbial biomass and activity, particularly in P-deficient soils, and this improvement was maintained for at least 70 days. Shifts in bacterial community composition were induced by a predominance of several specific taxonomic groups, all of which were capable of solubilizing P in soils. P-deficiency decreases the retention of exogenous labile C into soil. Adding labile C to P-deficient soils may shift P from relatively unavailable soil-bound pools into microbial biomass pools through pool cycling. Our results indicated negative effects of P-deficiency on soil C retention, as well as positive effects of labile C on soil P availability in arable soils.