Divergently expressed gene identification and interaction prediction of long noncoding RNA and mRNA involved in duck reproduction

- Young, first-laying, and old duck ovary is investigated by transcriptome sequencing.
- We found 2240 divergent transcription genes and 135 divergently expressed lncRNAs.
- Interaction of divergent lncRNAs and mRNAs in duck reproduction is predicted.
- We found 38 genes related to reproductive process and 6 genes are upregulated.
- We found 52 lncRNAs are closely correlated with genes related to reproduction.

Long noncoding RNAs (lncRNAs) and divergently expressed genes exist widely in different tissues of mammals and birds, in which they are involved in various biological processes. However, there is limited information on their role in the regulation of normal biological processes during differentiation, development, and reproduction in birds. In this study, whole transcriptome strand-specific RNA sequencing of the ovary from young ducks (60 days), first-laying ducks (160 days), and old ducks, i.e., ducks that stopped laying eggs (490 days) was performed. The lncRNAs and mRNAs from these ducks were systematically analyzed and identified by duck genome sequencing in the three study groups. The transcriptome from the duck ovary comprised 15,011 protein-coding genes and 2905 lncRNAs; all the lncRNAs were identified as novel long noncoding transcripts. The comparison of transcriptome data from different study groups identified 2240 divergent transcription genes and 135 divergently expressed lncRNAs, which differed among the groups; most of them were significantly downregulated with age. Among the divergent genes, 38 genes were related to the reproductive process and 6 genes were upregulated. Further prediction analysis revealed that 52 lncRNAs were closely correlated with divergent reproductive mRNAs. More importantly, 6 remarkable lncRNAs were correlated significantly with the conversion of the ovary in different phases. Our results aid in the understanding of the divergent transcriptome of duck ovary in different phases and the underlying mechanisms that drive the specificity of protein-coding genes and lncRNAs in duck ovary.