|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|5520479||1544902||2017||8 صفحه PDF||سفارش دهید||دانلود کنید|
Present study evaluates antioxidative and erythrocyte membrane stabilizing potential of methanol extract of Carissa carandas leaves Cc(L)M and its purified fraction CM 1. Phytochemically Cc(L)M revealed presence of alkaloid, steroids, saponins and tanins. The results of antioxidant assays of Cc(L)M and CM 1 found to be dose dependent. However, in TLC bioautography, CM 1 showed yellow coloured scavenged band of DPPH reagent. The results revealed that CM 1 has significant (pâ¤0.05) free radical scavenging potential compared to Cc(L)M. The purified fraction CM 1 was found to be most potent with lowest EC50 values for DPPH (546.4Â Âµg/ml), OH- (498.5Â Âµg/ml) radical scavenging and 57.48Â Âµg/ml for reducing power assay. It also erythrocytes membrane stabilization effect with lowest EC50 1.31Â mg/ml as compared to Cc(L)M (2.56Â mg/ml) and standard diclofenac sodium (1.00Â mg/ml). The FT-IR and GC-HRMS analysis of CM 1 reveal the presence of steroid derivative 20-hydroxypregnan 18-oic acid.
Journal: Biocatalysis and Agricultural Biotechnology - Volume 10, April 2017, Pages 216-223