کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5522284 | 1545904 | 2017 | 4 صفحه PDF | دانلود رایگان |
- EHS matrices such as Matrigel or Geltrex can absorb antibiotics.
- Viability of bacteria cultured in this setting is diminished.
- Retention of antibiotics by EHS matrix interferes with cell infection experiments.
In studying the interaction between bacteria and host cells in vitro, the latter are frequently cultured on commercially available biotic matrices such as Matrigel® or Geltrex®. To avoid contamination, host cells are commonly grown in the presence of antibiotics. However, we present here the finding that cell culture on such a matrix in the presence of antibiotics interferes with the outcome of subsequent infection experiments by virtue of diminished bacterial survival. By comparing outcomes for penicillin-susceptible and resistant strains of Staphylococcus aureus, we show that residual penicillin remains in the matrix despite the antibiotics' withdrawal from culture. Hence, the use of antibiotics should be avoided in this context.
Journal: Journal of Microbiological Methods - Volume 139, August 2017, Pages 103-106