کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5522315 1545906 2017 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Dynamic light scattering: A fast and reliable method to analyze bacterial growth during the lag phase
ترجمه فارسی عنوان
پراکندگی نور پویا: یک روش سریع و قابل اطمینان برای تجزیه و تحلیل رشد باکتری در طول مرحله عقب است
کلمات کلیدی
پراکندگی نور پویا، مرحله لاگین رشد، شمارش صفحات، اشریشیا کولی، استافیلوکوک اورئوس،
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
چکیده انگلیسی


- Real time monitoring of microorganism growth during the lag phase is important.
- Dynamic light scattering provides viable count in the lag and exponential phases.
- Dynamic light scattering provides details of the lag-to-exponential phase transition.
- A comparison between plate counting and dynamic light scattering is reported.

A comparison between plate counting (PC) and dynamic light scattering (DLS) is reported. PC is the standard technique to determine bacterial population as a function of time; however, this method has drawbacks, such as the cumbersome preparation and handling of samples, as well as the long time required to obtain results. Alternative methods based on optical density are faster, but do not distinguish viable from non-viable cells. These inconveniences are overcome by using DLS. Two different bacteria strains were considered: Escherichia coli and Staphylococcus aureus. DLS was performed at two different illuminating conditions: continuous and intermittent. By the increment of particle size as a function of time, it was possible to observe cell division and the formation of aggregates containing very few bacteria. The scattered intensity profiles showed the lag phase and the transition to the exponential phase of growth, providing a quantity proportional to viable bacteria concentration. The results revealed a clear and linear correlation in both lag and exponential phase, between the Log10(colony-forming units/mL) from PC and the Log10 of the scattered intensity Is from DLS. These correlations provide a good support to use DLS as an alternative technique to determine bacterial population.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiological Methods - Volume 137, June 2017, Pages 34-39
نویسندگان
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