|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|5523024||1546064||2018||5 صفحه PDF||سفارش دهید||دانلود کنید|
- FSH combined with LH is effective on IVM of zebrafish oocyte.
- FSH combined with LH may be applied to IVM of other teleost.
- Pituitary concentration used in zebrafish IVM was dose-dependent.
- FSH combined with LH result in zebrafish live larvae after IVF.
This study aimed to assess the effects of carp pituitary extract (CPE), follicle stimulating hormone (FSH) and luteinizing hormone (LH) on zebrafish oocyte maturation and the ability of these mature oocytes to be fertilized and developed until hatching. Stage III follicles were matured in eight treatments: five concentrations of CPE (16, 32, 48, 64 and 80Â Î¼g/mL), one of FSH (0.5Â Î¼g/mL), one of LH (0.5Â Î¼g/mL), or one combination of FSH (0.5Â Î¼g/mL) and LH (0.5Â Î¼g/mL). Maturation rates in CPE treatments were 12.8% (16Â Î¼g/mL), 24.8% (32Â Î¼g/mL), 27.0% (48Â Î¼g/mL), 22.7% (64Â Î¼g/mL) and 9.6% (80Â Î¼g/mL); in FSH was 15.7% (0.5Â Î¼g/mL), in LH was 31.8% (0.5Â Î¼g/mL) and in FSH (0.5Â Î¼g/mL) combined with LH (0.5Â Î¼g/mL) it was 50.4%. InÂ vitro fertilization was performed in all treatments; however, only the treatment combining FSH and LH resulted in fertilized oocytes. After maturation using FSH combined with LH, the cleavage rate was 33.3% and hatching rate of live larvae was 20.0%. These results showed that FSH combined with LH was effective in IVM of zebrafish oocyte.
Journal: Theriogenology - Volume 105, 1 January 2018, Pages 115-119