|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|5523027||1546064||2018||8 صفحه PDF||سفارش دهید||دانلود کنید|
- A supplemented vitrification protocol with l-carnitine is proposed.
- l-carnitine was used to improve post-warming survival and quality of inÂ vivo-produced ovine embryos.
- Although metabolic function, LC did not improve survival rates and quality parameters.
- LC was efficient to improve quality of ovine embryos at a molecular level, by altered CrAT and PRDX1 expression.
l-carnitine is an antioxidant and Î²-oxidation stimulator substance commonly used to improve metabolic performance of oocytes and embryos in inÂ vitro systems. However, few studies have evaluated its beneficial effects in embryos produced inÂ vivo. This study aimed to evaluate the effect of l-carnitine supplementation into vitrification or warming solutions on the post-warming character of day 6-7 inÂ vivo-produced ovine embryos. l-carnitine (3.72Â mM) was added to vitrification (Experiment 1) or warming solutions (Experiment 2). In experiments 1 and 2, the embryos were vitrified using straw and cryo-tip protocols, respectively. InÂ vitro culture (IVC) of warmed embryos was performed for 72Â h in order to evaluate survival rates, reactive oxygen species (ROS) levels, total cell number (TCN), number of apoptotic cells, apoptotic index evaluation, and gene expression analysis of carnitine palmitoyltransferase I and 2 (CPT1 and CPT2), carnitine O-acetyltransferase (CrAT), and peroxiredoxin-1 (PRDX1). In experiment 1, survival rate, ROS levels after 24Â h of IVC, total cell number at 24Â h and 72Â h, apoptotic cells and apoptotic index at 72Â h of IVC were similar in embryos vitrified in medium supplemented with LC or not. Gene expression analysis showed no differences in CPT1 and CPT2 mRNA relative abundance in embryos of both experiments compared to fresh embryos (FE); however, CrAT was downregulated (pÂ <Â 0.05) in C1, and PRDX1 was downregulated (pÂ <Â 0.05) in both the control (C1) and l-carnitine (LC1) groups, compared to FE. Moreover, CrAT and PRDX1 were upregulated (pÂ <Â 0.05) in C2, and CrAT was downregulated (pÂ <Â 0.05) in LC2, in relation to FE. Although the short-term LC supplementation at 3.72Â mM did not improve survival, and quality parameters of inÂ vivo-produced ovine embryos, it could affect their quality at a molecular level. In conclusion, further investigations with different concentrations of LC and tools are needed for improvement of the efficiency of these strategies.
Journal: Theriogenology - Volume 105, 1 January 2018, Pages 150-157