|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|5523116||1546079||2017||5 صفحه PDF||سفارش دهید||دانلود کنید|
The aim of the present study was to evaluate whether repeated ovum pick-up (OPU) procedures may affect the efficiency of OPU programs for inÂ vitro embryo production (IVEP) in Bos indicus (Nelore) donors. In addition, the repeatability (r) efficiency of IVEP was also assessed. Data available were from 432 OPU-IVEP sessions that were performed at random stages of the estrous cycle in 36 cycling, nonlactating Nelore donors. Semen from three Nelore bulls was used for the IVF. Donors were submitted to 12 consecutive OPU procedures, with an interval of approximately 30Â days between sessions. Data were analyzed as repeated measures using the GLIMMIX procedure of SAS 9.3. Cows yielding â¥15 COCs were defined as “high” COCs and cows with less than 15 COCs were defined as “low” COCs donors. The number of COCs retrieved decreased over time in donors classified with high COCs and remained fairly steady in cows with low COCs at the beginning of the program (Psession*COC retrieved groupsÂ =Â 0.02). Moreover, the number of COCs retrieved (PÂ <Â 0.0001), and number of blastocysts produced per OPU (PÂ =Â 0.001) was greater for the high COCs donors compared with the low COCs category, and these results were consistent across OPU sessions. Interestingly, there was no effect of COCs category on the rate of blastocyst development (PÂ =Â 0.83). In addition, number of blastocysts produced were not affected by repeated OPU (PÂ =Â 0.37) and interactions between time and COC category (PÂ =Â 0.72). Similarly, blastocyst rate was not affected by repeated OPU (PÂ =Â 0.21) and interactions between time and COC category (PÂ =Â 0.58). Despite of COC category of the donor cows, repeatability was high for the number of COCs retrieved (rÂ =Â 0.81), number of blastocysts produced per OPU (rÂ =Â 0.79), and blastocyst rate (0.69). In conclusion, overall numbers of COCs decreased over time in donors classified as having high COCs. However, cumulative amounts of produced blastocysts were greater in donors with high COCs. More importantly, high repeatability was observed in terms of IVEP efficiency. Therefore, IVEP programs can be significantly improved with more aggressive selection toward donors with greater numbers of COCs.
Journal: Theriogenology - Volume 90, 1 March 2017, Pages 54-58