|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|5523385||1401372||2017||5 صفحه PDF||سفارش دهید||دانلود کنید|
As the taste receptor for monosodium glutamate (umami) is expressed in both murine and human spermatozoa and the presence of Î±-gustducin and Î±-transducin, G proteins involved in the umami taste signaling, has been described in boar germ cells, the aim of this study was to evaluate if monosodium glutamate (MSG) would exert any effect on sperm-oocyte binding, inÂ vitro fertilization (IVF) and sperm parameters during inÂ vitro induced capacitation.For sperm-zona pellucida binding assay, boar spermatozoa were preincubated for 1Â h and then coincubated for 1Â h with denuded inÂ vitro matured oocytes in presence of different concentrations of MSG (0, 0.1, 1, 10Â mM). MSG 1 and 10Â mM significantly (PÂ <Â 0.05) increased the mean number of sperm bound to ZP compared with control (12.3Â Â±Â 9.0, 17.8Â Â±Â 11.3, 17.6Â Â±Â 10.8, MSG 0, 1 and 10Â mM respectively).For inÂ vitro fertilization trials, both sperm preicubation (1Â h) and gamete coincubation (1Â h) were performed in presence of different concentrations of MSG (0, 0.1, 1, 10Â mM). After 19Â h of culture in fresh IVF medium, oocytes were fixed. MSG 1Â mM significantly (PÂ <Â 0.05) increased the penetration rate compared with control (53.7Â Â±Â 20.4 vs. 36.8Â Â±Â 16.2).The addition of MSG during inÂ vitro induced capacitation of boar spermatozoa did not cause any significant difference, compared with control, on the percentage of viable cells, spermatozoa with intact acrosome and the percentage of spermatozoa displaying tyrosine-phosphorylation of sperm tail proteins.In order to evaluate whether the effect elicited by MSG could be due to glutamate uptake in boar spermatozoa, fertilization trials were performed in presence of either 1Â mM MSG or 1Â mM MSGÂ +Â 100Â Î¼M DL-threo-beta-hydroxyaspartic acid (THA), a non selective inhibitor of glutamate uptake. A significant increase (PÂ <Â 0.05) in the penetration rate in both MSG and MSGÂ +Â THA groups compared to control was recorded (39.8Â Â±Â 15.7, 53.7Â Â±Â 22.1, 52.2Â Â±Â 23.7, Control, MSG and MSGÂ +Â THA respectively) while no difference in penetration rate between MSG and MSGÂ +Â THA treatment was observed suggesting that sperm glutamate transporters are not involved in the pathway mediating this effect.Our study demonstrates for the first time that glutamate exerts a positive effect on sperm-oocyte binding and fertilization. Further studies are needed to clarify the mechanism by which glutamate exert his effect.
Journal: Theriogenology - Volume 95, June 2017, Pages 149-153