کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5523505 1546076 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cobalamin supplementation during in vitro maturation improves developmental competence of sheep oocytes
ترجمه فارسی عنوان
مکمل کوبالامین در طی بلوغ انسانی در شرایط آزمایشگاهی، توانایی رشد در تخمک های گوسفند را بهبود می بخشد
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
چکیده انگلیسی


- Cobalamin supplementation during in vitro maturation (IVM) enhances ovine oocyte quality.
- Cobalamin supplementation improves DNA methyltransferases expression in IVP placentae.
- Cobalamin supplementation during IVM ameliorates vasculogenesis in early placentae.

Pregnancies obtained by Assisted Reproductive Technologies are at higher risk of miscarriage than those obtained naturally. Previously, we reported impaired placental vascular development of in vitro produced (IVP) sheep embryos and defective DNA methylation in the placentae of those embryos. One reason behind these observed defects may be an impaired One Carbon Metabolism (OCM) The present study was performed to test the hypothesis that Cobalamin (Vitamin B12, an important OCM co-factor) supplementation during IVM corrects DNA methylation of IVP embryos and, consequently, ameliorates placental vasculogenesis. To this aim, embryos derived from oocytes matured with Cobalamin (B12 group) or without (negative control group, −CTR) were transferred to synchronized recipient sheep. At day 20 of pregnancy, collected embryos were morphologically evaluated while placentae were subjected to qPCR and histological analysis. The positive control group (+CTR) consisted of conceptuses obtained from naturally mated sheep. Results showed an increased fertilization rate in the B12 group vs -CTR (69.56% vs 57.91% respectively, P = 0.006) not associated with quantitative improvement in blastocyst and/or implantation rate (44.32% vs 36.67% respectively, P > 0.05). Moreover, Cobalamin supplementation during oocyte IVM ameliorated resulting conceptuses quality, in terms of placental vascularization (vessels' maturity and vasculogenetic factors' expression). The expression of DNA methyltransferases (DNMT1, DNMT3A and DNMT3B) was also improved in placentae from the B12 group. In conclusion, Cobalamin supplementation during oocyte IVM improves IVP embryo quality. These results suggest that Cobalamin should be included in standard IVM media.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Theriogenology - Volume 93, 15 April 2017, Pages 55-61
نویسندگان
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