کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5526882 1401554 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
miR-429 inhibits metastasis by targeting KIAA0101 in Soft Tissue Sarcoma
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
miR-429 inhibits metastasis by targeting KIAA0101 in Soft Tissue Sarcoma
چکیده انگلیسی


- miR-429 inhibits metastasis by targeting KIAA0101 in Soft Tissue Sarcoma.
- miR-429 suppresses proliferation, migration, anchorage independent growth and invasion in HT1080.
- We validated targeting of miR-429 and KIAA0101 by reporter assay.
- KIAA0101 overexpression is involved in decreased survival of STS patients.

Soft tissue sarcomas (STS) are a heterogeneous group of rare tumors with high metastatic potential. There being only a handful of publication on metastasis of STS, we investigated the miRNA mediated target gene regulations in modulating the metastatic processes in this cancer. In this study, we amalgamated gene and miRNA expression profiles of high-grade STS samples with miRNA target predictions and identified miR-429 targeting KIAA0101 as a novel pair, which remain unexplored in STS metastasis. We validated their expression in metastatic fibrosarcoma cell line, HT1080 and performed several functional assays using miRNA mimics and KIAA0101 over-expression vector to confirm their role in metastasis. We observed miR-429 is downregulated in HT1080 cells and acting as an anti-metastatic miRNA that inhibited proliferation, migration, anchorage independent growth and invasion by de-repressing KIAA0101. Moreover, the renilla luciferase reporter assay confirmed that miR-429 targets KIAA0101 by binding to its 3/UTR and influence its expression. Taken together, our work demonstrated miR-429 mediates deregulation of KIAA0101 by acting as an anti-metastatic miRNA that targets KIAA0101 pro-metastatic gene during metastasis of STS.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 357, Issue 1, 1 August 2017, Pages 33-39
نویسندگان
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