کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5527179 | 1401568 | 2017 | 10 صفحه PDF | دانلود رایگان |
- Mesenchymal stem cells increase precursors of M2-macrophages under hypoxia.
- ICAM-1/LFA-1 axis plays an important role on the M2-poralization.
- Novel co-culture system was established to supply a number of M2-macrophages.
Immunosuppressive/anti-inflammatory macrophage (MÏ), M2-MÏ that expressed the typical M2-MÏs marker, CD206, and anti-inflammatory cytokine, interleukin (IL)-10, is beneficial and expected tool for the cytotherapy against inflammatory diseases. Here, we demonstrated that bone marrow-derived lineage-positive (Lin+) blood cells proliferated and differentiated into M2-MÏs by cooperation with the bone marrow-derived mesenchymal stem cells (MSCs) under hypoxic condition: MSCs not only promoted proliferation of undifferentiated M2-MÏs, pre-M2-MÏs, in the Lin+ fraction via a proliferative effect of the MSCs-secreted macrophage colony-stimulating factor, but also promoted M2-MÏ polarization of the pre-M2-MÏs through cell-to-cell contact with the pre-M2-MÏs. Intriguingly, an inhibitor for intercellular adhesion molecule (ICAM)-1 receptor/lymphocyte function-associated antigen (LFA)-1, Rwj50271, partially suppressed expression of CD206 in the Lin+ blood cells but an inhibitor for VCAM-1 receptor/VLA-4, BIO5192, did not, suggesting that the cell-to-cell adhesion through LFA-1 on pre-M2-MÏs and ICAM-1 on MSCs was supposed to promoted the M2-MÏ polarization.Thus, the co-culture system consisting of bone marrow-derived Lin+ blood cells and MSCs under hypoxic condition was a beneficial supplier of a number of M2-MÏs, which could be clinically applicable to inflammatory diseases.
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Journal: Experimental Cell Research - Volume 358, Issue 2, 15 September 2017, Pages 411-420