کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5528467 1547965 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization of microbiome in bronchoalveolar lavage fluid of patients with lung cancer comparing with benign mass like lesions
ترجمه فارسی عنوان
تشخیص میکروبیومی در مایع لاروی برونکوه آلوئولار بیماران مبتلا به سرطان ریه نسبت به توده خوش خیم مانند ضایعات
کلمات کلیدی
سرطان ریه، توده خوش خیم، مایع برونکوآلوئولار، میکروبیوم، توالی نسل جدید،
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
چکیده انگلیسی


- Microbiota in lung cancer were investigated in BAL fluid.
- Microbiota in lung cancer were compared with benign lung mass.
- The genera Veillonella and Megasphaera have the potential to become a biomarker in lung cancer.

ObjectivesDisruption in the stability of respiratory microbiota is known to be associated with many chronic respiratory diseases. However, only few studies have examined microbiomes in lung cancer. Therefore, we characterized and compared the microbiomes of patients with lung cancer and those with benign mass-like lesions.Materials and methodsBronchoalveolar fluid was collected prospectively to evaluate lung masses in patients who had undergone bronchoscopies from May to September 2015. Twenty-eight patients (20 male, 8 female) were enrolled: 20 diagnosed with lung cancer and 8 diagnosed with benign diseases. Samples were analysed by 16S rRNA-based next-generation sequencing.ResultsThe participants' mean age was 64 ± 11 years. Bacterial operational taxonomic units were classified into 26 phyla, 44 classes, 81 orders, 153 families, 288 genera, and 797 species. The relative abundance of two phyla (Firmicutes and TM7) was significantly increased in patients with lung cancer (p = 0.037 and 0.035, respectively). Furthermore, two genera (Veillonella and Megasphaera) were relatively more abundant in lung cancer patients (p = 0.003 and 0.022, respectively). The area under the curve of a combination of these two genera used to predict lung cancer was 0.888 (sensitivity = 95.0%, specificity = 75.0% and sensitivity = 70.0%, specificity = 100.0%; p = 0.002).ConclusionThe results indicate that differences exist in the bacterial communities of patients with lung cancer and those with benign mass-like lesions. The genera Veillonella and Megasphaera showed the potential to serve as biomarkers to predict lung cancer. Thus, the lung microbiota may change the environment in patients with lung cancer.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Lung Cancer - Volume 102, December 2016, Pages 89-95
نویسندگان
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